HPLC Principle:
High Performance Liquid Chromatography (HPLC) is used for applications in the analytical chemistry, biochemistry and many other industries. The HPLC separates mixtures by identifying the components. The components of the HPLC system may included solvent organizer, pumps, samplers, column and detector. The pump is used to move the solvent through the column and the detector analyzes the components. Some of the general configurations of HPLC systems are shown below.
The components of the HPLC system may include solvent organizer, pumps, samplers, column and detector. The pump is used to move the solvent through the column and the detector analyzes the components. Some of the general configurations of HPLC systems are shown below. Our machines are very customizable. To have a complete HPLC system, you would need a delivery system, sampler, detector, workstation, column and solvent organizer. You also have the option to add a column oven if you like. Delivery system options include Isocratic, Binary or Quaternary pumps. The sampler includes manual and auto samplers. Detector is a UV-VIS instrument while the solvent organizer has three different models that can be used. You can choose from our preconfigured machines or customize one.
HPLC Standards:
Instrument Standard: GB/T 16631-2008 general rules of HPLC
Agricultural and food field: Sulfonamide residues GB 29694-2013, Veterinary drug residues GB/T21317-2007, Aflatoxin GB/T 30955-2014, Vitamin A GB/T 17817-2010, Melamine migration GB 31604.15-2016
Pharmaceutical field: Geranium GB/T 22251-2008, Panax notoginseng 2008
Environment: N-methylcarbamate insecticide EPA 531, benzidine compounds in wastewater EPA 605, polycyclic aromatic hydrocarbons EPA 8310
Cosmetic field: Niacin, nicotinamide GB/T29664-2013, antibiotics GB/T 24800.1-2009, preservatives GB/T 35800-2018, colorants GB/T 30927-2014
HPLC Application:
Pharmaceutical Analysis
- Determination of soybean Isoflavone content in Soybean Isoflavone Soft Capsule with TT- HPLC system with core-shell column.
Environment Protection
- Content determination of 9 phenols compounds by TT- HPLC system. The results show that the method is accurate and sensitive.
Food Safety
- Simultaneously determining the content of 8 kinds of organic acids and Vitamins in fruit with TT-HPLC Diode Array Detector.
Agriculture
Simultaneously determining the content of 4 kinds of sulfa-drugs in fodder by using TTHPLC Diode Array Detector.
Chemical
- Simultaneously determining the content of 6 kinds of UV absorbers in cosmetics.
Antibiotics
Determining the content of terramycin, doxycycline, tetracycline and auromycin by TT- HPLC system.
HPLC System Components:
High pressure constant current pump
- Lower noise, higher reliability
- This upgrade continues the classic reciprocating parallel pump design, featuring high flow accuracy and low pressure pulsation
- The original one-way valve design consists of a valve core body, a gem ball seat and a gem ball to form an integral structure
- Upgrade to add plunger rod automatic retracting function, you can easily replace the seal ring without opening the case
- Extend the warranty time and provide a more comprehensive after-sale service
- For users who use the buffer salt system, a pump with post-column cleaning function is specially introduced
Complete liquid phase configuration
Compatible analysis and preparation requirements for semi-preparative liquid chromatography systems
Binary high pressure system quaternary gradient system
Suitable for different of gradient blends
The binary high-pressure system with high-precision parallel pumps fully guarantees the stability of the infusion and can meet the accuracy requirements of high-pressure gradient analysis.
The quaternary gradient system is equipped with a fully imported four-channel online degassing and proportional solenoid valve, which can easily realize the replacement, mixing and cleaning of multiple mobile phases, and the operation is more convenient
UV 100-Vis detector
This upgrade of the UV100 detector introduces the Wufeng Instruments ultra-high pressure, ultra-fast liquid chromatograph design concept to minimize distortion due to system errors.
Intelligent ARCUS 5 autosampler
Arcuss comes with its own control software, which is fully computer-operated, simple and convenient, and extremely easy to use. It can quickly and easily per[1]form sample analysis, dilution, mixing and other operating functions. The use of jewel rods as the active metering pump, the service life of the sealing ring can reach more than 1 million times, and the loss of metering accuracy and the trouble of replacement caused by the wearable parts to the user are mini[1]mized. Under normal use conditions, it does not need to be replaced for 3 to 5 years.
Column thermostat
The integrated design of the temperature control part and the solvent tray is adopted, the function is more practical, the temperature control is more stable, and the temperature control range is 5℃-80℃ above room tempera[1]ture. Reproducibility of retention time is good. Two chromatographic columns can be placed at the same time, which can satisfy the serial analysis of chromatographic columns. The system is all controlled by digital anti-con[1]trol technology, and all operations can be completed on the computer
Evaporative Light Scattering Detector
The evaporative light scattering detector is a universal detector. The ELSD universal detection method eliminates the common difficulties in traditional HPLC detection methods. Unlike ultravio[1]let and fluorescence detectors, the response of ELSD does not depend on the optical characteristics of the sample, and any volatility Samples below the mobile phase can be detected without being affected by their functional groups. The detection of all compounds except low boiling point compounds is now commonly used to detect substances without UV absorption.
